Custom Antibody Development, Genemed Synthesis Inc

Genemed Synthesis is dedicated to provide quality service to our clients. Our custom service team provides multiple choices to meet the unique requirements of our client’s polyclonal and monoclonal developments. We provide the options of antigen (peptide, protein, biologic, or small molecule) or preferred species (rabbit, chicken, mouse or other big animal) to one of our 70-day conventional protocols for production of high quality antisera. Subsequent peptide-specific purification and antibody characterization is also an option to meet our client’s needs.

Genemed Synthesis will make every effort to provide the customer with this custom service in the shortest possible time. A period of three to nine months will normally be required for completion of the project. Delays may be incurred if the antigen is poorly immunogenic or produces unstable clones.

The success of the project is dependent upon the material used, supplied by either the customer or by our supplier. Genemed Synthesis does not guarantee that the monoclonal antibodies produced will work in the client's own application. The client is responsible for determining the utility of these antibodies in their specific applications. Customer must do further testing to ensure applicability.

All clones, cell lines, antibodies and other material which are the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.

Please contact us for a quotation of your customized monoclonal development project.

Service Items	Price
Polyclonal antibody production, Complete service	$1050/project
Phospho-peptide polyclonal antibody production and phospho-peptide affinity purification, Complete service	$2,370/project
Peptide synthesis	$15/AA
Phospho-peptide synthesis	$120/site
Peptide conjugation to KLH	$200/peptide
Immunization and antisera production without peptide synthesis	$550/antigen
Titer test by ELISA	$100/project
Western blotting (WB) test	$200/project
Antibody purification by protein G	$500/project
Antibody purification by peptide affinity column	$700/project
Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column	$1,400/project
Polyclonal antibody production: additional rabbit	$175/rabbit
Polyclonal antibody production: extended protocol	$275/rabbit
Monoclonal antibody production, Complete service	$8,000/project
Phospho-specific monoclonal antibody production, Complete service	$8,500/project
Monoclonal antibody production: peptide conjugation to KLH	$200/peptide
Monoclonal antibody production: immunization and antisera production	$2,000/antigen
Monoclonal antibody production: hybridoma fusion and screening of clones	$3,000/fusion
Monoclonal antibody production: screening of phospho-specific clones	$2,000/fusion
Monoclonal antibody production: Stabilization of clones and expansion of clones. Relevant clones(single cells) are frozen at this stage and hybridoma medium(500-100ml) collected for further studies	$2000/project
Monoclonal antibody production: Isotyping of clones and ascites production. Isotypes of all selected clones will be determined and production of ascites from 5 mices if needed	$1500/project
Monoclonal antibody production: large scale culture up to 200 ml	$500/clone
Monoclonal antibody service: ascites production up to 20 ml	$500/clone
Monoclonal antibody service: antibody purification by protein G	$500/clone