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Profiler array offers chemiluminescent
detection for high-throughput gene expression profiling, so
you can focus on your experiments instead of worrying about safety
issues and costly radioactive disposal procedures.
FAST Exposure times
Profiler's digoxigenin-labeled probes are just as sensitive as radioactively
labeled probes. And because exposure times are much shorter than
with radioactive probes, you get results in minutes, not days.
Efficient & selective hybridization
We guarantee that each spot on the Star Profiler Array is free of
homologous sequences, so you get efficient and selective hybridization
with your labeled probe. Star Profiler is the only commercially
available array made with long oligonucleotides, which we carefully
design to make sure every sequence is unique.
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Figure 1.
The actual size of the Profiler array is 7.5cm X 2cm.
The small size of the Profiler array allows for easy handling.
Each membrane contains two sets of Profiler Arrays. Thus
experiments can be done in duplicates if needed.
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Focus on key genes
The Profiler Array is a nylon membrane that contains genes that
play significant roles in several pathways and are corroborated
with published literature. We've specially designed Star Profiler
to make high-throughput expression profiling of cancer or diseased
tissues relatively easy. Star Profiler Array contains genes from
six biologically significant groups: Oncogenes & Tumor Suppressors,
Cell Cycle, Apoptosis, Kinases, Cytokines & Receptors, and Regulatory
Proteins. In addition, we our developing targeted arrays devoted
to specific areas of research. Currently we offer the Oncogene Profiler
Array, which contains 295 human oncogenes and tumor suppressors.
Other targeted arrays, such as the Apoptosis Profiler Array will
be available in 2001.
Benefits of the Profiler Array
Unlike other macroarrays, which often require poly-A+ RNA, the Profiler
Array has no preference for total or poly-A+ RNA. In fact, our method
provides equivalent results using either total RNA or mRNA, so there
is no need for special RNA purification procedures.
After cDNA labeling, Profiler Arrays are hybridized overnight with
DIG-11-dUTP-labeled cDNA. The next day, the array membranes are
washed and subsequently incubated with Anti-DIG-Alkaline Phosphatase
(DIG-AP) Antibody. Upon addition of the CDP-Star chemiluminescent
substrate (provided), detection of DIG-AP can be obtained within
minutes by exposing array membranes to x-ray film (Figure 1 & 2)
or by using a chemiluminescent-imaging system, such as Alpha Innotech's
FluorChem Imaging System (Figure 3).
Each Profiler Array Nonradioactive System includes two membrane
arrays; solutions for hybridization and washing, random primer and
dNTP Mix for cDNA probe synthesis, and stripping; control RNA; and
chemiluminescent detection reagents. Profiler arrays can be purchased
separately and are provided with hybridization and array buffer,
as well as washing, and stripping solutions. A complete gene list,
including full names and GenBank accession numbers, are provided
with each purchase.
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