Large mRNAs are notoriously difficult to convert into full-length cDNA. In conventional PCR-based methods, the inherent inefficiency of the reverse transcriptase enzyme results in very few large genes in the final PCR product. At Genemed, we have developed a technology that bypasses this limitation. We designed the patented Gene Enrichment Technology to specifically enrich for large, full-length transcripts in the final cDNA preparation. Thus, researchers can rapidly and easily amplify almost any target transcript-regardless of its size.

Each Full-Length cDNA is tissue-specific, double-stranded cDNA that has already been pre-enriched for large transcripts (Figure 1). You can immediately get results with this ready-to-use cDNA-just run a simple PCR and visualize your results by agarose gel electrophoresis.

GSI Full-Length cDNAs are ideal for amplifying your gene of interest using gene-specific primers. Alternatively, you can profile expression of your gene of interest by using GSI Full-Length cDNAs prepared from different tissues.


Figure. The large titin gene (7.0 kb) was successfully amplified using Human GSI Full-Length cDNA generated from different tissues; however, the titin gene was not amplified using other commercially available, non-enriched cDNA. Lanes A: GSI cDNA. Lanes B: Competitor, non-enriched cDNA. Lane M: 1-kb DNA ladder.


 

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