GSI risoRNA Isolation Reagent is a ready-to-use reagent designed for the isolation of total RNA from animal tissues or cells. A single bottle of RISO reagent is sufficient to isolate RNA from 25-50g of tissue. RISO provides the researcher with a highly effective method to recover total RNA from both small and large quantities of tissue. RNA can be recovered from as little as 5-10mgs of tissue. The simple protocol allows for simultaneous sample processing and the entire process takes a little less than an hour to be completed. The high quality yield of total RNA is perfect for many downstream applications such as RT-PCR, cDNA library generation, cloning and other molecular biology applications.

RISO RNA Isolation Reagent is a ready-to-use reagent designed for the isolation of total RNA from tissue or cells. RISO is a phenol, guanidine and ammonium based reagent and yields high quality RNA for downstream applications such as RT-PCR, cDNA library construction, cloning and other molecular biology applications. RISO Reagent provides the researcher with a highly effective method to recover total RNA from both small and large quantities of tissue. RNA can be recovered from as little as 5-10 mgs of tissue. A single bottle of RISO reagent is sufficient to isolate RNA from 25-50g of tissue. The simple protocol allows for simultaneous sample processing and the entire process takes a little less than an hour to be completed. The resulting purified RNA is free from protein and DNA contamination.

Figure. Analysis of total RNA isolated from 10 mg samples of different human tissues using RISO Reagent. Three micrograms of RNA were loaded per lane on a 1.2% agarose formaldehyde gel. Lane 1, Human Fetal Lung total RNA. Lane 2, Human Fetal Liver total RNA. Lane 3, Human Adult Gastric total RNA. Lane 4, Human Placenta total RNA. Lane M: 0.24 - 9.7 kb RNA ladder.